Immunofluorescence analysis of Cytokeratin Pan was performed using 70% confluent log phase A-431 cells. The cells were fixed and permeabilized with ice-cold 100% acetone and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin Pan Monoclonal Antibody (B311.1) (Product # MA1-91326) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoskeletal localization. The images were captured at 60X magnification.